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The needle used to draw from the BAC water vial should not be the same needle used to inject — drawing blunts the tip slightly
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These persister cells exhibit a reversible, non-genetic phenotypic trait unrelated to heritable drug resistance mutations, with their progeny restoring wild-type antifungal susceptibility, and the biofilm-specific niche is confirmed to be essential for their formation (LaFleur et al., 2006)
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