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For in vitro studies, researchers typically reconstitute with bacteriostatic water (0.9% benzyl alcohol) or, for cell-culture protocols, with sterile water or PBS at concentrations matched to the assay
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[38] used this model to study cardiac physiology, employing a new video recording method coupled with an isotonic transducer for the three-dimensional study of the heart and intracardiac valves, as well as their possible response to the application of different cholinergic neurotransmitters
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