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Solution B contained the components of the L-glutamate detection system: 238 mM triethanolamine (TEA), 30.1 mM K 2 HPO 4 pH 7.4, Triton X-100 (1.5% v/v), sodium azide (0.013% w/v), 3.85 mM NAD + , 275.1 mM iodonitrotetrazolium (INT), 6.25 mUnits/μL diaphorase, and 12.5 mUnits/μL glutamate dehydrogenase (GDH) (Megazyme, Wicklow, Ireland)

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