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He noticed something remarkable during his research: when aged liver tissue was exposed to GHK-Cu, it began synthesizing proteins in patterns that resembled much younger tissue
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After washing twice with PBS to remove unbound dye, the green (JC-1 monomers) and red (JC-1 aggregates) fluorescence ratio that monitored the proportion of mitochondrial depolarization was immediately acquired on a flow cytometer by following the manufacturer’s protocol
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